New reverse genetics and transfection methods to rescue arboviruses in mosquito cells

by Atieh et al.

In order to overcome potential technological issues related to the study of arboviruses, the authors of this study designed an improved DNA transfection protocol for insect cells and then demonstrated that the simple and flexible ISA (Infectious Subgenomic Amplicons) reverse-genetics method can be efficiently applied to both mammalian and mosquito cells to generate in days recombinant infectious positive-stranded RNA viruses belonging to genera Flavivirus (Japanese encephalitis, Yellow fever, West Nile and Zika viruses) and Alphavirus (Chikungunya virus). 

27th October 2017 • comment